Study on apoptosis in HL-60 cells and its related gene regulation induced by enriched uranium
Creators
- 1. Suzhou Univ., Suzhou (China). Inst. of Radiological Medicine
Description
Objective: To study characteristics of injury in apoptosis of HL-60 cells and its related genes bcl-2 and bax expression induced by enriched uranium 235U. Methods: DNA gel electrophoresis was used to show the apoptosis of HL-60 cells. Immunohistochemical technique was used to identity the expression of bcl-2 and bax proteins in HL-60 cells, meanwhile RNA dot blot hybridization was used to show the expression of bcl-2 mRNA in HL-60 cells. Results: HL-60 cells irradiated with 235U displayed characteristics of DNA ladder formation in apoptotic cells. While the expression of bcl-2 protein was as high as (88±7)% in unirradiated control cells, down-regulation [lowered to (61±5)%] of its expression was noted after exposure to 235U. The expression of bax protein was low in control cells ,and after irradiation it did not change obviously. A marked down-regulation of bcl-2 mRNA expression was found in irradiated HL-60 cells. Conclusion: Progression of apoptosis in HL-60 cells induced by 235U is dependent on the time elapse of 235U irradiation. The apoptotic action of 235U in HL-60 cells is associated with down-regulation of the apoptosis-related gene bcl-2
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 23
- Journal Issue
- 1
- Journal Page Range
- p. 17-19
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 36021257
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- APOPTOSIS; BIOLOGICAL RADIATION EFFECTS; DATA COVARIANCES; DNA DAMAGES; ELECTROPHORESIS; ENRICHED URANIUM; GELS; GENES; HELA CELLS; HISTOLOGICAL TECHNIQUES; IMMUNOASSAY; INTERNAL IRRADIATION; LEUKEMIA; MESSENGER-RNA; STATISTICS; TIME DEPENDENCE; TRANSCRIPTION; URANIUM 235
- Descriptors DEC
- ACTINIDE NUCLEI; ACTINIDES; ALPHA DECAY RADIOISOTOPES; ANIMAL CELLS; BIOASSAY; BIOLOGICAL EFFECTS; COLLOIDS; DISEASES; DISPERSIONS; ELEMENTS; EVEN-ODD NUCLEI; HEAVY NUCLEI; IMMUNE SYSTEM DISEASES; INTERNAL CONVERSION RADIOISOTOPES; IRRADIATION; ISOMERIC TRANSITION ISOTOPES; ISOTOPE ENRICHED MATERIALS; ISOTOPES; MATERIALS; MATHEMATICS; METALS; MINUTES LIVING RADIOISOTOPES; NEOPLASMS; NUCLEI; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIOISOTOPES; RNA; SPONTANEOUS FISSION RADIOISOTOPES; TUMOR CELLS; URANIUM; URANIUM ISOTOPES; YEARS LIVING RADIOISOTOPES