The evaluation of split renal function in the experimentally induced transient renal vein obstruction

The split renal function was measured in order to evaluate the alteration of renal function secondary from transient renal vein obstruction. Three microcuries of I-131 Hippuran and 7 μCi of Tc-99m DTPA were injected into the femoral vein of adult male rats 30 min, 3 hr, 6 hr, 2 days and 1 week after the renal vein obstruction for 30 min. Blood samples were taken from the tail vein 5, 10, 15, 20 and 20 min after injection. Immediately after taking blood sample at 30 min, both kiendys and urinary bladder with ureters were taken. The radioactivities of Tc-99m and I-131 of these samples were measured respectively using a well type scintillation counter at the energy range of 140 KeV±25% and 360 KeV±25%. In the obstructed side, the blood clearances of I-131 Hippuran (ERPF: effective renal plasma flow) and Tc-99m DTPA (GFR: glomerular filtration rate) were decreased at 30 min and 6 hr after the release of left renal vein obstruction. Therefore, the filtration fraction was not significantly changed. In conclusion, the single study using either Tc-99m DTPA or I-131 Hippuran may be enough to evaluate the alteration of split renal function in a model of renal vein obstruction, althought the combined study using both agents is more preferable. (author)