Application of nuclear magnetic resonance spectroscopy of human serum to leukaemia clinical study

To study metabolism of phospholipid and lipoprotein and lactic acid by nuclear magnetic resonance spectroscopy, and investigate the feasibility in leukaemia clinical study, serum sample of leukaemia patients and healthy controls were collected and measured with MSL-300 MHz spectrometer. The phospholipid contents were known by contrast relatively integral area of PC signal and (PE + SM) signal with 31P-NMRS. A lineshape fitting model was used to analyze various lipoprotein and lactic acid changes by methyl and methylene groups in 1H-NMR spectrum of leukaemia serum. In spectra of serum of leukaemia patients, the phospholipid signals strength were significantly decreased. It reflected lower phosphatidylcholine and phosphatidylcholine and sphingomyelin content in serum of leukemia patients, especially for original un-treatment patient. The various lipoprotein contents were difference and lactic acid was significantly higher than normal control. NMR technique is a convenience method. It can primely reflect the difference of phospholipid and lipoprotein and lactic acid between leukaemia patients and normal control from molecule level. It provided a new method for study of leukaemia and is worthy to further study