Nuclear resonant vibrational spectroscopy and its application to the biological research. Probe for the active center of enzymes

Nuclear resonant vibrational spectroscopy is a site-selective technique allowing the observation of only the vibrational modes of Fe in the active center of the enzymes, which makes it ideal for studying active center without interference from protein backbone modes. Many iron-dependent enzymes including hydrogenase, which catalyse both production and consumption of H₂, have a close relation to the energies, environments, medical and so on. The principle and features of the nuclear resonant vibrational spectroscopy and its application to the hydrogenase and halogenase is introduced. The structures and electronic states of there active centers and its relation to the activities were elucidated. (author)