Microbial degradation of multiple PAHs by a microbial consortium and its application on contaminated wastewater

Highlights: • The consortium PDMC is capable of effectively biodegrading several PAHs and heterocyclic derivatives. • A novel metabolite 1,2-dihydroxy-4a,9a-dihydroanthracene-9,10-dione of anthracene degradation was identified. • The various degradation pathways of consortium PDMC were proposed based on the identified intermediates. • The consortium PDMC significantly facilitates small-scale PAHs-contaminated wastewater bioremediation. Polycyclic aromatic hydrocarbons (PAHs) are widely distributed in the environment and pose a serious threat to human health. Due to their unfavorable biological effects and persistent properties, it is extremely urgent to effectively degrade PAHs that are present in the environment, especially in wastewater. In this study, we obtained an efficient bacterial consortium (PDMC), consisting of the genera Sphingobium (58.57−72.40%) and Pseudomonas (25.93−39.75%), which is able to efficiently utilize phenanthrene or dibenzothiophene as the sole carbon source. The phenanthrene-cultivated consortium could also degrade naphthalene, acenaphthene, fluorene, anthracene, fluoranthene, benzo[a]anthracene, dibenzofuran, carbazole and indole, respectively. Furthermore, we identified the multiple key intermediates of aforementioned 11 substrates and discussed proposed pathways involved. Notably, a novel intermediate 1,2-dihydroxy-4a,9a-dihydroanthracene-9,10-dione of anthracene degradation was detected, which is extremely rare compared to previous reports. The PDMC consortium removed 100% of PAHs within 5 days in the small-scale wastewater bioremediation added with PAHs mixture, with a sludge settling velocity of 5% after 10 days of incubation. Experiments on the stability reveal the PDMC consortium always has excellent degrading ability for totaling 24 days. Combined with the microbial diversity analysis, the results suggest the PDMC consortium is a promising candidate to facilitate the bioremediation of PAHs-contaminated environments.