Detection of inflammatory sites by In-111-oxine labeled neutrophils

Neutrophil labeling was performed by using recently developed 111In-oxine complex. 111In-oxine-labeled neutrophils injected into the patients with inflammation were significantly accumulated in the infected area. 11In could label neutrophils with the advantages of higher labeling efficiency, normal viability due to phagocytosis index and trypan blue exclusion test, and simple labeling procedure. Neutrophil survival labeled with 111In-oxine in a normal patient was 7.0 hours of T-1/2 and that in a neutropenic patient was 1.0 hour, in which higher radioactivity was detected in the collected pus 24 hours after the infusion. This technique would be utilized not only in the detection of inflammatory sites, but in simultaneous observation of neutrophil survival and organ distribution in the field of neutrophilic kinetic studies. (author)