Spectrophotometric method for Determination of sulfamerazine Using 2,4-dinitrophenylhydrazineReagent

This research describes the spectrophotometric method for determination of sulfamerazine (SMZ) in aqueous solution. This study involves the use 2,4-dinitrophenylhydrazine (2,4-DNPH) as a coupling reagent to determine sulfamerazine using potassium periodate (KIO₄) as an oxidative agent in alkaline medium. The coupling produces an orange product with maximum absorption at 489 nm, the concentration range of sulfamerazine solution (2.5-55) μg.ml⁻¹within limits of beer law with molar absorptivity of 9251 L.mole⁻¹.cm⁻¹ and Sandel index 0.0286 μg.cm⁻². The accuracy and precision of this research was examined by calculating the percentage of recovery and the percentage of the relative standard deviation and it was shown that they ranged between 98.55 - 99.77. sulfamerazine has been successfully determined in pharmaceuticals using the proposed method by a standard addition method and two levels of concentration. (paper)