The micronucleus assay with human B-lymphocytes after staining with acridine orange for biological radiation dosimetry
Creators
- 1. Radiation Health Research Institute, Seoul (Korea, Republic of)
- 2. National Institute of Radiological Sciences, Chiba (Japan)
Description
The frequency of micronuclei in cytokinesis-blocked human peripheral blood lymphocytes from 5 healthy volunteers was studied after in vitro irradiation with 137 Cs γ -ray in dose ranges of 0 to 50 cGy. After sorting out and separating the B-lymphocytes, the frequency of radiation induced micronuclei was observed as the end point markers for the biological radiation dosimetry after stain with Giemsa and Acridine orange dyes. The micronuclei frequency in B-lymphocytes was significantly elevated from 10 to 30 cGy γ -irradiation. The determination of micronuclei in B-lymphocytes after stain with Acridine orange was higher than those of Giemsa. The frequency of micronuclei in B-lymphocytes was observed at least two times higher than those of T-lymphocytes, respectively. Therefore, the determination of low dose radiation induced micronuclei in B-lymphocytes after staining with Acridine orange is likely to have the greatest potential in the estimation of radiation exposure
Additional details
Publishing Information
- Publisher
- KARP
- Imprint Place
- Taejon (Korea, Republic of)
- Imprint Title
- Proceedings of the first Asian and Oceanic Congress for Radiation Protection(AOCRP-1)
- Imprint Pagination
- [1 CD-ROM]
- Journal Page Range
- [8 p.]
Conference
- Title
- 1. Asian and Oceanic Congress for Radiation Protection(AOCRP-1)
- Dates
- 20-24 Oct 2002
- Place
- Seoul (Korea, Republic of)
INIS
- Country of Publication
- Korea, Republic of
- Country of Input or Organization
- Korea, Republic of
- INIS RN
- 36053972
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Resource subtype / Literary indicator
- Conference, Non-conventional Literature
- Descriptors DEI
- ACRIDINE ORANGE; BLOOD; DOSIMETRY; GAMMA RADIATION; IN VITRO; LYMPHOCYTES; LYMPHOKINES; RADIATION DOSES
- Descriptors DEC
- ACRIDINES; AMINES; ANIMAL CELLS; AROMATICS; AZAARENES; AZINES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DOSES; DYES; ELECTROMAGNETIC RADIATION; GROWTH FACTORS; HETEROCYCLIC COMPOUNDS; IONIZING RADIATIONS; LEUKOCYTES; MATERIALS; MITOGENS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PROTEINS; PYRIDINES; RADIATIONS; SOMATIC CELLS
Optional Information
- Notes
- 26 refs, 3 figs, 1 tab