Published January 1979 | Version v1
Journal article

Sensitive radioimmunoassay for somatostatin using N-[125I]-tyr-somatostatin as labelled antigen

  • 1. CHUL, Quebec (Canada). Lab. of Molecular Endocrinology and Service of Gastro-Enterology

Description

A sensitive radioimmunoassay for somatostatin using N-(125I]-Tyr-somatostatin is described and compared with using [125I]-Tyr1-somatostatin. The minimum detectable amount of somatostatin using N-[125I]-Tyr-somatostatin as tracer was 0.1 to 0.5 pg, which is approximately 10-fold lower detection limit of the RIA using [125I]-Tyr1-somatostatin. Moreover, it was found that the shelf-life of N-[125I]-Tyr-somatostatin was prolonged in comparison with labelled Tyr1-somatostatin. Human pancreatic and gastric extracts displayed immunological similarity to synthetic somatostatin tetradecapeptide. (author)

Additional details

Publishing Information

Journal Title
Clin. Endocrinol.
Journal Volume
10
Journal Issue
1
Series
Clin. Endocrinol.
Journal Page Range
47-54
ISSN
0300-0664