Published 1983 | Version v1
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Detection of deletion mutations at the gpt locus in pSV2gpt transformed CHO cells

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Description

Deletion mutations were detected at the gpt locus in HGPRT-deficient CHO cells transformed by the pSV2gpt plasmid vector. The purine analog, 6-thioguanine (TG), is metabolized by XGPRT and was shown to be applicable for the isolation of mutants at the gpt locus in one pSV2gpt transformed CHO cell line carrying a single copy of the gpt gene. Induction of TG/sup r/ mutants in this cell line by x-rays was quantifiable. Specific deletions of pSV2gpt sequences in two TG/sup r/ mutants were detected by blot hybridization. The sites of these depletions provide a molecular basis for the observed TG/sup r/ phenotype. 17 references

Availability note (English)

MF available from INIS under the Report Number; Available from NTIS, PC A02/MF A01; 1 as DE83015766.

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Additional details

Publishing Information

Imprint Pagination
12 p.
Report number
DOE/NBM--3015766

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
15009121
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Numerical Data
Descriptors DEI
ANIMAL CELLS; EXPERIMENTAL DATA; GENES; HAMSTERS; MUTATIONS; OVARIES; PLASMIDS; RADIATION EFFECTS; X RADIATION
Descriptors DEC
ANIMALS; BODY; CELL CONSTITUENTS; DATA; ELECTROMAGNETIC RADIATION; FEMALE GENITALS; GONADS; INFORMATION; IONIZING RADIATIONS; MAMMALS; NUMERICAL DATA; ORGANS; RADIATIONS; RODENTS; VERTEBRATES