Adenosine triphosphate-pyrophosphate isotopic exchange reaction: a tool for determination of tryptophan
Creators
Description
Quantitative determination of tryptophan at the picomole level is described, using the ATP-[32P]PPi isotopic exchange reaction catalyzed by tryptophanyl-tRNA synthetase. Sensitivity limits of 500 fmol were obtained. The presence of other amino acids at a 1000-fold excess over tryptophan did not interfere significantly with the quantitative determination of tryptophan. The specificity of the reaction was checked using five tryptophan analogs. These analogs did not prevent the determination of tryptophan when present in the same concentration range as tryptophan. When sensitive determination of a single amino acid is needed, the ATP-[32P]PPi exchange reaction catalyzed by aminoacyl-tRNA synthetases is suggested as a general method and as an alternative to HPLC procedures
Additional details
Publishing Information
- Journal Title
- Anal. Biochem.
- Journal Issue
- no.2
- Series
- Anal. Biochem.
- ISSN
- 0003-2697
- CODEN
- ANBCA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18018471
- Subject category
- S60: APPLIED LIFE SCIENCES; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
- Descriptors DEI
- AMINO ACIDS; ATP; CATALYSIS; CHROMATOGRAPHY; ISOTOPIC EXCHANGE; PHOSPHORUS 32; PYROPHOSPHATES; QUANTITATIVE CHEMICAL ANALYSIS; TRYPTOPHAN
- Descriptors DEC
- AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CHEMICAL ANALYSIS; DAYS LIVING RADIOISOTOPES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; INDOLES; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PHOSPHORUS ISOTOPES; PYRROLES; RADIOISOTOPES; SEPARATION PROCESSES