Microarray analysis of micro-ribonucleic acid expression in primary immunoglobulin A nephropathy
Creators
- 1. Clinical Medical Research, The Second Clinical Medical College, Shenzhen People's Hospital, Guangdong Province (China)
- 2. Kidney Transplantation and Hemo Purification Center, Hospital of Guilin, Guangxi Province (China)
- 3. Key Laboratory of The Laboratory Medical Diagnostics, Ministry of Education, Chongging Medical Univ. (China)
Description
Objective was to explore the relationship between immunoglobulin A nephropathy (IgAN) and microRNA (miRNA). We analyzed the miRNA expression profiles in renal biopsies from 11 IgAN patients and 3 controls at the Kidney Transplantation and Hemo Purification Center of 181 Hospital, China, from May to October 2007, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. This study identified 132 miRNAs in renal samples of, of which 35 miRNAs up regulated in igAN biopsies. The chip results were confirmed by northern blot analysis and by quantitative real time polymerase chain reaction (RT-PCR) tests. Our study may help clarify the molecular mechanisms involved in the pathogenesis of IgAN potentially serve as a novel diagnostic biomarker of IgAN. (author)
Additional details
Publishing Information
- Journal Title
- Saudi Medical Journal
- Journal Volume
- 29
- Journal Issue
- 10
- Journal Page Range
- p. 1388-1393
- ISSN
- 0379-5284
INIS
- Country of Publication
- Saudi Arabia
- Country of Input or Organization
- Saudi Arabia
- INIS RN
- 41094326
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- BIOPSY; CHINA; IMMUNITY; IMMUNOGLOBULINS; MEDICINE; PATHOGENESIS; PATIENTS; POLYMERASE CHAIN REACTION; PROTEINS; RENAL CLEARANCE
- Descriptors DEC
- ASIA; CLEARANCE; DIAGNOSTIC TECHNIQUES; EXCRETION; GENE AMPLIFICATION; GLOBULINS; ORGANIC COMPOUNDS; PROTEINS