Catalytic activity of nuclease P1: Experiment and theory
- 1. Pacific Northwest Lab., Richland, WA (United States)
- 2. Roswell Park Cancer Institute, Buffalo, New York (United States)
Description
Nuclease P1 from Penicillium citrinum is a zinc dependent glyco-enzyme that recognizes single stranded DNA and RNA as substrates and hydrolyzes the phosphate ester bond. Nuclease Pl seems to recognize particular conformations of the phosphodiester backbone and shows significant variation in the rate of hydrolytic activity depending upon which nucleosides are coupled by the phosphodiester bond. The efficiency of nuclease Pl in hydrolyzing the phosphodiester bonds of a substrate can be altered by modifications to one of the substrate bases induced by ionizing radiation or oxidative stress. Measurements have been made of the effect of several radiation induced lesions on the catalytic rate of nuclease Pl. A model of the structure of the enzyme has been constructed in order to better understand the binding and activity of this enzyme on various ssDNA substrates
Availability note (English)
MF available from INIS under the Report Number; Also available from OSTI as DE95004956; NTIS; US Govt. Printing Office Dep.Files
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Additional details
Publishing Information
- Imprint Pagination
- 9 p.
- Report number
- PNL-SA--25462
Conference
- Title
- relationships at early times.
- Acronym
- International workshop on radiation damage in DNA
- Dates
- 1-6 Oct 1994.
- Place
- Gleneden, OR (United States).
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 26049920
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- BIOCHEMICAL REACTION KINETICS; BIOLOGICAL RADIATION EFFECTS; FUNGI; GENE MUTATIONS; NUCLEASES; PROTEIN STRUCTURE; RADIATION INDUCED MUTANTS
- Descriptors DEC
- BIOLOGICAL EFFECTS; ENZYMES; ESTERASES; HYDROLASES; KINETICS; MUTANTS; MUTATIONS; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; PLANTS; PROTEINS; RADIATION EFFECTS; REACTION KINETICS
Optional Information
- Contract/Grant/Project number
- Contract AC06-76RL01830
- Funding organization
- USDOE, Washington, DC (United States).
- Secondary number(s)
- CONF-9410280--1.