Toxicities of polystyrene nano- and microplastics toward marine bacterium Halomonas alkaliphila

Highlights: • Toxicities of plastics debris were closely related with their primary particle sizes. • Increased extracellular polymeric suggested the possible protective mechanisms. • Reactive oxygen species might be responsible for the toxicities of plastics debris. • Nanoplastics can interrupt the ecological function of Halomonas alkaliphila. Nano- and microplastics have been shown to cause negative effects on marine organisms. However, the toxicities of nano- and microplastics toward marine bacteria are poorly understood. In this study, we investigated the toxic effects of polystyrene nano- and microplastics on the marine bacterium Halomonas alkaliphila by determining growth inhibition, chemical composition, inorganic nitrogen conversion efficiencies and reactive oxygen species (ROS) generation. The results showed that both nano- and microplastics inhibited the growth of H. alkaliphila in high concentrations, while nanoplastics rather than microplastics influenced the growth inhibition, chemical composition and ammonia conversion efficiencies of H. alkaliphila at concentration of 80 mg/L. The ROS generation indicated oxidative stress induced by nano- but not microplastics, and the oxidative stress induced by nanoplastics may provide a significant effect on bacteria. Furthermore, the positively charged nanoplastics (amine-modified 50 nm) induced higher oxidative stress toward bacteria than that induced by negatively charged nanoplastics (non-modified 55 nm). The increased extracellular polymeric substances as evidenced by transmission electron microscope (TEM) observation suggested the possible bacterial protective mechanisms. The present study illustrates for the first time the impact of plastics debris on the inorganic nitrogen conversion efficiencies of marine bacteria. Our findings highlight the effects of microplastics on the ecological function of marine organisms.