Published May 1, 1990
| Version v1
Journal article
A sensitive radioisotopic method for the measurement of NAD(P)H: Its application to the assay of metabolites and enzymatic activities
Description
A radioisotopic method for the assay of NADH or NADPH is presented, which is based on the conversion of 2-[U-14C]ketoglutarate to 14C-labeled glutamate in the reaction catalyzed by glutamate dehydrogenase. The efficiency of the method is close to 75%, its precision (coefficient of variation) close to 5%, and its sensitivity close to 0.1 pmol/sample. This simple and rapid method can be applied to the measurement of several metabolites and enzymatic activities. In the present study, its application to the assay of sorbitol, 3-hydroxybutyrate, glutamate dehydrogenase, 3-hydroxybutyrate dehydrogenase, and glyceraldehyde-3-phosphate dehydrogenase is documented
Additional details
Publishing Information
- Journal Title
- Analytical Biochemistry
- Journal Volume
- 186
- Journal Issue
- 2
- Series
- Anal. Biochem.
- Journal Page Range
- 236-242
- ISSN
- 0003-2697
- CODEN
- ANBCA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 21086616
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ENZYME ACTIVITY; GLUTAMIC ACID; METABOLITES; NADH2; NADP; OXIDOREDUCTASES; RADIOASSAY; RATS; TRACER TECHNIQUES
- Descriptors DEC
- AMINO ACIDS; ANIMALS; CARBOXYLIC ACIDS; COENZYMES; ENZYMES; ISOTOPE APPLICATIONS; MAMMALS; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; RODENTS; VERTEBRATES