Published 1992 | Version v1
Journal article Metadata-only

Radiolabelling of sperm cells with 99mTc-HM-PAO

Creators

  • 1. Orszagos Frederic Joliot-Curie Sugarbiologiai es Sugaregeszseguegyi Kutato Intezet, Budapest (Hungary)
  • 2. Allatorvostudomanyi Egyetem, Budapest (Hungary). Szueleszeti es Szaporodasbiologiai Tanszek es Klinika

Description

The study of the influence of labelling volume on the labelling efficiency showed decreased yield when the volume was increased. The survival rate was unchanged over 0.5 ml of reaction volume. During labelling procedure only a few cells (6%) survived in the incubation volume was less than 0.4 ml. Changing the incubation time the radiolabelling yield increased for 10 minutes, and thereafter practically did not change. The optimum conditions of sperm labelling yielded a high labelling efficiency (70-80%) and survival rate (50-60%). The 99mTc-HM-PAO labelled sperm cells seem to be suitable to study the in vivo and in vitro sperm transport. (author) 14 refs.; 5 figs.; 2 tabs

Additional details

Additional titles

Subtitle (English)
Investigations to determine the optimium conditions for LEUCO-SCINT kit
Original title (Hungarian)
Ondosejtek jelzese
Original subtitle (Hungarian)
A jelzesi koeruelmenyek optimalizalasa LEUCO-SCINT keszlethez.

Publishing Information

Journal Title
Itd. Izotoptechnika, Diagnosztika
Journal Volume
35
Journal Issue
3
Journal Page Range
p. 91-96.
ISSN
0865-0497
CODEN
IZDIE2

INIS

Country of Publication
Hungary
Country of Input or Organization
Hungary
INIS RN
25005292
Subject category
S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
Resource subtype / Literary indicator
Numerical Data
Descriptors DEI
EFFICIENCY; LABELLING; SPERMATOZOA; STABILITY; STATISTICAL DATA; TECHNETIUM 99
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DATA; GAMETES; GERM CELLS; HOURS LIVING RADIOISOTOPES; INFORMATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; NUCLEI; NUMERICAL DATA; ODD-EVEN NUCLEI; RADIOISOTOPES; TECHNETIUM ISOTOPES; YEARS LIVING RADIOISOTOPES