Radioprotective and cytotoxic functions of nanoparticles, metal complexes and alkene

Most synthetic molecules currently under study for clinical and commercial applications exhibit little to extreme undesirable consequences on the organisms in addition to specific effects. These effects are determined by the nature and dosage of the compound. Nanoparticles and metal complexes have been put to use here for utilizing their potential to kill eukaryotic and prokaryotic cells in vitro culture systems. In addition, radioprotective properties of an alkene derivative molecule (AD) have been investigated in depth here and subsequently one may visualize its potential to serve these requirements. Different biochemical assays, SOD/SDH activity and GSH were measured using spectrophoto/fluorometric methods. SDH was estimated using PMS/DCIP, electron acceptors. Prokaryotic and eukaryotic cell culture technique: includes antioxidant enzyme assay, cytotoxicity assays. Metallic nanoparticles exerted cytotoxic action on eukaryotic cells. Different species of E Coli showed increase in zone of inhibition with increasing concentration of metal. Significant cytotoxic effect of synthetic Platinum complex on eukaryotic cells was also observed. Following exposure of lipid bilayers preparations to different gamma radiation doses, different types of damages were observed. Presence of AD lead to significant inhibition of radiation induced changes. Peroxidised products reduced while loss of SDH/SOD activities were reduced. Cytotoxic potency of the nanoparticles increased gradually as the net content of metal component increased. Moreover, significant reduction in radiation induced loss of glutathione, activity of SDH and SOD was exerted by AD, emphasizing its radio-protecting role. Further exploration using other systems would get an insight into its versatile role and side effects if any. Because of their instant therapeutic effect, synthetic preparations are even now preferred over plant preparations. (author)