Published July 24, 2006 | Version v1
Journal article Metadata-only

Purification, crystallization and preliminary X-ray crystallographic analysis of rice bifunctional α-amylase/subtilisin inhibitor from Oryza sativa

Creators

  • 1. Life Science Group, Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076,Taiwan (China)
  • 2. Institute of Bioinformatics and Structural Biology, National Tsing-Hua University, Hsinchu 30013,Taiwan (China)
  • 3. Department of Hospitality Management, Nan Jeon Institute of Technology, Yen-Shui, Tainan 73746,Taiwan (China)
  • 4. Department of Physics, National Tsing-Hua University, Hsinchu 30013,Taiwan (China)

Description

The crystallization of rice α-amylase/subtilisin bifunctional inhibitor is reported. Rice bifunctional α-amylase/subtilisin inhibitor (RASI) can inhibit both α-amylase from larvae of the red flour beetle (Tribolium castaneum) and subtilisin from Bacillus subtilis. The synthesis of RASI is up-regulated during the late milky stage in developing seeds. The 8.9 kDa molecular-weight RASI from rice has been crystallized using the hanging-drop vapour-diffusion method. According to 1.81 Å resolution X-ray diffraction data from rice RASI crystals, the crystal belongs to space group P21212, with unit-cell parameters a = 79.99, b = 62.95, c = 66.70 Å. Preliminary analysis indicates two RASI molecules in an asymmetric unit with a solvent content of 44%

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309106023335; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242909

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242909;
DOI
10.1107/S1744309106023335;
PII
S1744309106023335;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
62
Journal Issue
Pt 8
Journal Page Range
p. 743-745
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46065448
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CRYSTALLIZATION; CRYSTALS; DIFFUSION; MOLECULAR WEIGHT; MOLECULES; RESOLUTION; SOLVENTS; SPACE GROUPS; SYNTHESIS; X-RAY DIFFRACTION
Descriptors DEC
COHERENT SCATTERING; DIFFRACTION; PHASE TRANSFORMATIONS; SCATTERING; SYMMETRY GROUPS

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2006
Notes
PMCID: PMC2242909; PMID: 16880545; PUBLISHER-ID: bw5152; OAI: oai:pubmedcentral.nih.gov:2242909