Published November 2011 | Version v1
Report Metadata-only

Characterization of heavy ion-induced damage in bacillus subtilis spores and their global transcriptional response during spore germination-role of B. subtilis's apurinic/apyrimidinic (AP) endonucleases in the resistance to heavy ion radiation

Creators

  • 1. German Aerospace Center, Cologne (Germany)
  • 2. National Inst. of Radiological Sciences, Chiba, Chiba (Japan)

Description

The proposed research project is aimed to provide new insights on the spore resistance to heavy ions and the effects on different linear energy transfer (LET)-charged HZE particles. With this project, spores of Bacillus subtilis 168, (wild-type and several selected DNA repairdeficient strains) were used for studying the microbial response heavy ions irradiation. DNA repair capabilities were investigated be the determination of the spore survivability and spore-specific protection mechanisms after irradiation. The activation of DNA repair genes were detected during germination by using DNA microarrays. For studying the DNA repair of treated spores during germination an integrated systems approach was used, id est (i.e.) all experiments were performed in a combination of various biochemical and molecular biological methods to study the spore resistance to heavy ion bombardment. (author)

Part of:
2010 annual report of the research project with heavy ions at NIRS-HIMAC

Additional details

Identifiers

URL
http://id.nii.ac.jp/1657/00073745/;

Publishing Information

Imprint Title
2009 annual report of the research project with heavy ions at NIRS-HIMAC
Imprint Pagination
322 p.
Journal Page Range
p. 116-117
Report number
NIRS-M--244

INIS

Country of Publication
Japan
Country of Input or Organization
Japan
INIS RN
53067172
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Non-conventional Literature
Descriptors DEI
BACILLUS SUBTILIS; BACTERIAL SPORES; DNA REPAIR; ENDONUCLEASES; GENETIC RADIATION EFFECTS; GERMINATION; HEAVY IONS; HELIUM IONS; IRON IONS; LET; MICROARRAY TECHNOLOGY; MUTATION FREQUENCY; STRAND BREAKS; X RADIATION
Descriptors DEC
BACILLUS; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BIOTECHNOLOGY; CHARGED PARTICLES; DNA DAMAGES; DNA-ASE; ELECTROMAGNETIC RADIATION; ENERGY TRANSFER; ENZYMES; ESTERASES; GENETIC EFFECTS; HYDROLASES; IONIZING RADIATIONS; IONS; MICROORGANISMS; NUCLEASES; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; PROTEINS; RADIATION EFFECTS; RADIATIONS; REPAIR; SPORES

Optional Information

Notes
This record replaces 45052105
Secondary number(s)
HIMAC--136