Lipopolysaccharide-stimulated interleukin-6 production for radioimmunoassay

Prepration of liquid phase radioimmunoassay (RIA) to assessment the interleukin-6 (IL-6) that produced in-house by microbial stimulation was the aim of the present study. The production of IL-6 through mouse and macrophage cell infections were caused by Escherichia coli lipopolysaccharide (LPS). Optimum dose of lipopolysaccharide was determined. Then, both ¹²⁵I- IL-6 tracer and polyclonal anti-IL-6 antibody were prepared. These reagents were used in detection of produced interleukin-6. Optimization and validation tests of local RIA were carried out. The results showed that the interleukin-6 produced by microbial macrophage or mouse infections gave positive qualitative detection using enzyme linked immunoassay sorbent assay. The local RIA was used in quantitative determination of local purified interleukin-6. (author)