Published April 1987 | Version v1
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Isolation of a XP-A cell clone with intermediate UV sensitivity following transfection with genomic mouse DNA. Isolation and characterization of transfected sequences

Creators

Description

The work presented here describes an attempt to restore repair proficiency in xp cells of the most UV sensitive complementation group A by DNA mediated gene transfer. The transfection conditions employed for the cell line used here (XP12Rotk-1) have been optimized. (orig./MG)

Availability note (English)

MF available from INIS under the Report Number.

Abstract (German)

In der vorliegenden Arbeit wurde versucht, den Reparaturdefekt von XP-Zellen der Komplementationsgruppe A, die die groesste UV-Sensitivitaet aufweisen, durch Gentransfer aufzuheben. In Vorexperimenten wurden die fuer die verwendete Zellinie (XP12ROtk-1) optimalen Transfektionsbedingungen etabliert. (orig./MG)

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Additional details

Additional titles

Original title (German)
Isolierung eines intermediaer UV-sensitiven Xeroderma Pigmentosum (Komplementationsgruppe A) Zellklons nach Transfektion genomischer Maus-DNA. Partielle Reklonierung und Charakterisierung der transfizierten Sequenzen

Publishing Information

Imprint Pagination
157 p.
Report number
KFK--4215

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
18089230
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Thesis
Descriptors DEI
BIOLOGICAL REPAIR; CELL CULTURES; CLONE CELLS; DNA; GENE RECOMBINATION; GENES; GENETIC ENGINEERING; GENOTYPE; MAN; MICE; MUTANTS; PHENOTYPE; RADIATION INJURIES; RADIOSENSITIVITY; ULTRAVIOLET RADIATION; XP CELLS
Descriptors DEC
ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BIOLOGY; DISEASES; ELECTROMAGNETIC RADIATION; GENETICS; INJURIES; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PRIMATES; RADIATION EFFECTS; RADIATIONS; RODENTS; VERTEBRATES