Published December 15, 1978 | Version v1
Journal article Metadata-only

A radiochemical method for the determination of transketolase activity in erythrocyte hemolysates

Creators

  • 1. Clinical Chemical and Haematological Laboratories, St. Elisabeth Ziekenhuis, Leiderdorp, Netherlands

Description

A radiochemical method for the determination of transketolase activity is reported. It is based on incubation of erythrocyte hemolysates with radio-active ribose 5-phosphate followed by isolation of sedoheptulose 7-phosphate on anion-exchange columns. The experimental conditions are discussed and the presence of phosphatase activity in the incubation mixture is demonstrated. The proposed method is compared with a current colorimetric method. Reference values are given for the transketolase activity and the thiamine pyrophosphate effect. (Auth.)

Additional details

Publishing Information

Journal Title
Clin. Chim. Acta
Journal Volume
90
Journal Issue
3
Series
Clin. Chim. Acta.
Journal Page Range
259-268
ISSN
0009-8981

INIS

Country of Publication
Netherlands
Country of Input or Organization
Netherlands
INIS RN
10441294
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
CARBON 14; ERYTHROCYTES; HEMOLYSIS; ION EXCHANGE CHROMATOGRAPHY; LABELLED COMPOUNDS; PHOSPHATASES; PYROPHOSPHATES; RADIOMETRIC ANALYSIS; RIBOSE; THIAMINE
Descriptors DEC
ALDEHYDES; AMINES; AZINES; AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; CARBON ISOTOPES; CHEMICAL ANALYSIS; CHEMICAL REACTIONS; CHROMATOGRAPHY; DECOMPOSITION; ENZYMES; ESTERASES; EVEN-EVEN NUCLEI; HETEROCYCLIC COMPOUNDS; HYDROLASES; HYDROXY COMPOUNDS; ISOTOPES; LIGHT NUCLEI; MONOSACCHARIDES; NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; OXYGEN COMPOUNDS; PENTOSES; PHOSPHORUS COMPOUNDS; PYRIMIDINES; QUANTITATIVE CHEMICAL ANALYSIS; RADIOISOTOPES; SACCHARIDES; SEPARATION PROCESSES; THIAZOLES; VITAMIN B GROUP; VITAMINS; YEARS LIVING RADIOISOTOPES