Transcriptome sequencing and analysis of functional genes in young leaves of bottle gourd

In order to explore the functional gene information of transcriptome in bottle gourd, the transcriptome of Fuzhou bottle gourd was sequenced by high-throughput non-parametric sequencing technology and 664252268 reads fragments were obtained. The reads gave 87518 Unigenes with the total length of 91405320 bp, and 76.03% of Unigenes were concentrated in 1-1000 bp. The results of functional annotations showed that 55725 Unigenes were annotated in Nr database, among which 26354 Unigenes were annotated in cucurbitaceae, accounting for 47.29% of the total Unigenes. GO database annotated 18278 Unigenes, which closely related to molecular function, cell structure function and biological process function with 55 subgroups. 41635 Unigenes were annotated in COG database, which were divided into information storage and processing, cell process and signal transmission, metabolism and non-characteristic genes with a total of 25 functional groups and the number of genes involved in amino acid transport and metabolism was the largest. There are 24770 Unigenes annotated in the KEGG database, involving 220 KEGG metabolic pathways. SSR locus retrieval results showed that there were 11029 SSR loci in 8 617 Unigenes containing SSR sequences, with a frequency of 9.846%. The results of this study would lay a foundation for the further development of metabolic pathways and SSR molecular markers in bottle gourd. (authors)