Two-monoclonal-antibody sandwich-type assay for thyrotropin, with use of an avidin-biotin separation technique
Creators
- 1. Univ. of Utah School of Medicine, Salt Lake City
Description
We have developed a sensitive, specific, noncompetitive, sandwich-type radioimmunoassay for human thyrotropin (hTSH), which can be performed in 30 min. The assay involves two monoclonal antibodies, selected for high affinity and specificity and also for reaction against antigenic sites on hTSH that are distal from each other. One of these antibodies is labeled with 125I; the other is conjugated covalently to biotin. Polystyrene beads were also conjugated covalently to biotin. After conjugation, the beads were incubated with avidin. These beads represent a rapid, simple method for separating hTSH-bound antibody from free antibody. The biotin-antibody-hTSH-125I-labeled antibody complexes bind to the beads and hTSH concentration is directly related to counts per minute. This assay can detect hTSH at a concentration of 0.06 milli-unit/L in serum
Additional details
Publishing Information
- Journal Title
- Clin. Chem. (Winston-Salem, N.C.)
- Journal Issue
- no.10
- Series
- Clin. Chem. (Winston-Salem, N.C.).
- ISSN
- 0009-9147
- CODEN
- CLCHA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18036705
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- BIOTIN; IODINE 125; MICE; MONOCLONAL ANTIBODIES; RADIOIMMUNOASSAY; TSH
- Descriptors DEC
- ANIMALS; ANTIBODIES; AZOLES; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HORMONES; IMIDAZOLES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOISOTOPES; RODENTS; TRACER TECHNIQUES; VERTEBRATES; VITAMIN B GROUP; VITAMINS