The use of uniform labeling with zinc-65 to measure stable zinc turnover in the mosquito fish, Gambusia affinis

Juvenile mosquitofish, Gambusia affinis, were reared in an environment uniformly labeled with 65Zn until they attained the specific activity of the environment. Retention of 65Zn in these fish was followed with time in a non-labeled environment. Analysis of the retention curve yielded three mathematical reservoirs of stable Zn each containing 8.72, 4.30 and 91.1% of the element with measured rate constants of 0.280, 0.0510 and 0.00295 days-1, respectively, unbiased by lack of uniform labeling of the organisms. (author)