Effect of oestrogen on prolactin receptors in porcine granulosa cells

The binding of 125I-labelled prolactin (PRL) to the porcine granulosa cells was examined. Displacement studies demonstrated that 125I-PRL was specifically bound to the granulosa cells. The specific binding of 125I-PRL to the granulosa cells decreased significantly as the follicles matured. The addition of diethylstilbestrol diphosphate enhanced the binding of 125I-PRL to the granulosa cells harvested from small, medium and large follicles. The results suggest that estrogen might have an important role in modulating PRL receptors in porcine granulosa cells. (author)