Published 1986 | Version v1
Miscellaneous Metadata-only

Preparation, purification and stability of high specific activity 125 sub(I) triiodonthyronine (T3)

Creators

  • 1. Instituto de Pesquisas Energeticas e Nucleares, Sao Paulo (Brazil)

Description

A radioiodination of triiodothyronine (T3) by modified chloramine-T method is described. Diiodothyronine (T2) was employed as the starting material, which yields 125I-T3 with specific activities of 110.8 + sub(-) 23.6 TBq/g. Purification by Sephadex G-25 fine column, elution with phosphate buffer in high pH has shown to be convenient, for the separation of the desired products. Studies on the stability of 125I-T3 stored in 50% solution of propylene glycol showed that it is stable for at least 3 moths. The T3 labelled was analysed, using three types of RIA: a) The PEG solution, b) the double antiserum with PEG and c) the solid phase technics. (Author)

Abstract (Portuguese)

Descreve-se a radioiodinacao do triiodotironina (T3) pela modificacao do metodo de cloramina-T. Usou-se o diiodotironina (T2) como material inicial, que produz 125I-T3 com atividades especificas de 110.8+ sub(-) 23.6 TBq/G. Verificou-se que a purificacao por coluna fina de Sephadex G-25, eluicao com tampao de fosfato de alto pH e conveniente para a separacao dos produtos desejados. Os estudos sobre a estabilidade de 125I-T3 estocado em 50% de solucao de propileno glicol mostraram que e estavel por pelo menos 3 meses. O T3 marcado foi analizado usando-se tres tipos de radioimunoensaio: a) solucao PEG, b) duplo soro imunizante com PEG e c) tecnicas de fase solida. (E.G.)

Additional details

Additional titles

Original title (no language)
Anais do 1. Congresso Geral de Energia Nuclear. v. 2.

Publishing Information

Imprint Title
Proceedings of the 1. General Congress of Nuclear Energy. v. 2
Imprint Pagination
443 p.
Journal Page Range
v. 2 p. 98-101.
Report number
INIS-BR--537(v.2)

Conference

Title
1. General Congress of Nuclear Energy.
Dates
17-20 Mar 1986.
Place
Rio de Janeiro, RJ (Brazil).

INIS

Country of Publication
Brazil
Country of Input or Organization
Brazil
INIS RN
17067605
Subject category
S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
Resource subtype / Literary indicator
Conference
Descriptors DEI
DIIODOTHYRONINE; IODINE 125; PURIFICATION; RADIOIMMUNOASSAY; STABILITY; TRIIODOTHYRONINE
Descriptors DEC
BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; PEPTIDE HORMONES; RADIOISOTOPES; THYROID HORMONES; TRACER TECHNIQUES

Optional Information

Notes
Imprint:Anais do 1. Congresso Geral de Energia Nuclear. v. 2.