An improved synthesis of [amino-15N]adenine; useful in the large scale synthesis of 2'-deoxy[amino-15N]adenosine

The synthesis of 15N labeled nucleic acids has seen increased utility for the probing of local interactions in polynucleotides by multinuclear and multidimensional nuclear magnetic resonance spectroscopy. Interest in these labeled molecules has been stimulated by new methods which allow for the generation of significant quantities of labeled nucleosides and, as a consequence, synthetic DNA oligomers. We have been interested in the construction of purine derived 2'-deoxynucleosides which are specifically labeled at multiple positions. To accomplish this task our approach required the synthesis of a large quantity of 2'-deoxy[amino-15N]adenosine, which we viewed as a common precursor, for a series of multiply labeled purine 2'-deoxynucleosides. (author)