Published 1978
| Version v1
Journal article
Immunogenicity of soluble extracts from a UV light-induced mouse sarcoma
Creators
- 1. Akademie der Wissenschaften der DDR, Berlin. Zentralinstitut fuer Krebsforschung
Description
Soluble tumor extracts were isolated from a UV light-induced mouse sarcoma by 3 M KCl extraction followed by 2 M (NH4)2SO4 precipitation. The precipitates dissolved in phosphate buffered saline (PBS) produced transplantation resistance against syngeneic cell inocula of the tumor at a restricted dose range. While 1.0-1.2mg of protein per mouse were effective, double this dose was not. An extract prepared from ascitic fluid of tumor-bearing mice showed effects similar to one prepared from tumor cells. (orig.)
Abstract (German)
Aus einem durch UV-Licht induzierten Maeusesarkom wurden durch 3 M KCl-Extraktion und 2 M (NH4)2SO4-Ausfaellung loesliche Tumorextrakte isoliert. Die in gepufferter Salzloesung (PBS) aufgeloesten Praezipitate bewirkten eine Transplantationsresistenz gegen syngeneische Zellinokula des Tumors in einem begrenzten Dosisbereich. Waehrend 1,0 - 1,2mg Protein je Maus wirksam waren, war die doppelte Dosis ineffektiv. Ein Extrakt aus Aszitesfluessigkeit von tumortragenden Maeusen zeigte aehnliche Wirkungen wie ein Extrakt aus Tumorzellen. (orig.)Additional details
Publishing Information
- Journal Title
- Cancer Immunol. Immunother.
- Journal Volume
- 3
- Journal Issue
- 4
- Series
- Cancer Immunol. Immunother.
- Journal Page Range
- 273-275
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 10448590
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- IMMUNE REACTIONS; IMMUNITY; IMMUNOLOGY; MICE; SARCOMAS; SOLVENT EXTRACTION; ULTRAVIOLET RADIATION
- Descriptors DEC
- ANIMALS; DISEASES; ELECTROMAGNETIC RADIATION; MAMMALS; NEOPLASMS; RADIATIONS; RODENTS; SEPARATION PROCESSES; VERTEBRATES