Development and characterization of nineteen new microsatellite markers (SSRs) for cinnamomum camphora (LAURACEAE) based on transcriptome sequencing

Cinnamomum camphora (Lauraceae) is an evergreen tree species with many essential oils that have been used in the fragrance, medicine and chemical industry. However, limited variation of existing microsatellite markers of C. camphor was found, which may hinder its applications in genetic diversity. In this study, newly developed microsatellite makers with dior trinucleotide motifs were isolated using the next-generation transcriptome sequencing approach. Nineteen polymorphic SSR loci of C. camphor were developed and characterized. The number of alleles (Na) varied from 1 to 7 per polymorphic locus for each population, and the observed and expected heterozygosities (Ho, He) ranged from 0.000 to 1.000 and from 0.000 to 0.964 respectively. Moreover, four microsatellites loci Cc-44459, Cc-94, Cc-68 and Cc-12 exhibited significant deviation from the Hardy-Weinberg equilibrium. Cross-species amplification showed that most of loci had the transferability in C. parthenoxylon and C. kanehirae, except for C. burmannii. The 19 newly developed microsatellite markers will be useful for exploring the population genetics of C. camphor and other species from the same genus. (author)