Published April 2009 | Version v1
Journal article Metadata-only

Mutagenesis of the somaclones in vitro of cut roses by 60Co γ-rays irradiation

Creators

  • 1. Supervision and Testing Centre for Flowers of Ministry of Agriculture, Kunming (China)
  • 2. Quality Standard and Testing Technology Inst., Yunnan Academy of Agricultural Sciences, Kunming (China)
  • 3. Flower Reearch Inst. of Yunnan Academy of Agricultural Sceicnes, Kunming (China)
  • 4. Flow Research Inst. of Yunnan Academy of Agricultural Sciences, Kunming (China)

Description

Mutagenesis of cut rose in vitro irradiated by 60Co γ-rays was studied. The callus of leaves and regenerations adventitious bud was used as the explants for mutagenesis. Effect of 60Co γ-rays irradiation on the callus's regeneration rate, adventitious bud's multiplication rate and vegetal status were studied. The results showed that the regeneration frequency of callus was decreased by 60Co γ-rays irradiation. The regenerations adventitious bud was the better experimental materials compared with the callus of leaves. The lethal dose was 122 Gy and the semi-lethal dose was 76 Gy according to the regression equation. The appropriate dose on adventitious bud by irradiation rays was 50-60 Gy. (authors)

Additional details

Publishing Information

Journal Title
Acta Agriculturae Nucleatae Sinica
Journal Volume
23
Journal Issue
2
Journal Page Range
p. 239-243
ISSN
1000-8551

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
41095593
Subject category
S60: APPLIED LIFE SCIENCES; S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
ADVENTITIOUS BUD TECHNIQUE; BUDS; COBALT 60; FLOWERS; GAMMA RADIATION; IN VITRO; IRRADIATION; LEAVES; LETHAL RADIATION DOSE; MUTAGENESIS; ORNAMENTAL PLANTS; RADIOINDUCTION; REGENERATION; REGRESSION ANALYSIS; VEGETATIVE PROPAGATION
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CLONING; COBALT ISOTOPES; DOSES; ELECTROMAGNETIC RADIATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LETHAL DOSES; MATHEMATICS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; PLANTS; RADIATION DOSES; RADIATIONS; RADIOISOTOPES; STATISTICS; YEARS LIVING RADIOISOTOPES

Optional Information

Notes
4 figs., 1 tab., 13 refs.