Published April 1999
| Version v1
Journal article
Radioiodination of recombinant human growth hormone and its use in radioimmunoassay
- 1. Board of Radiation and Isotope Technology (BRIT), Calcutta (India). Unit of Dept. of Atomic Energy
Description
Radioimmunoassay (RIA) of human growth hormone (hGH) using 125I-labelled tracer prepared from DNA recombinant hGH (r-hGH) and characterization of the tracer in the assay system are described. The radioiodination of r-hGH resulted in high yield of immunoreactive tracer. The immunoreactive fraction could be purified by gel-filtration on sephadex G-75. The quality of radioiodinated tracer of r-hGH has been found to be same as that of the tracer obtained from pituitary hGH (p-hGH) with respect to immunoreactivity, assay sensitivity and RIA standard curve parameters. (author)
Additional details
Publishing Information
- Journal Title
- Journal of Radioanalytical and Nuclear Chemistry
- Journal Volume
- 240
- Journal Issue
- 1
- Journal Page Range
- p. 343-347
- ISSN
- 0236-5731
- CODEN
- JRNCDM
INIS
- Country of Publication
- Hungary
- Country of Input or Organization
- Hungary
- INIS RN
- 30022132
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- CHEMICAL PREPARATION; IODINE 125; LABELLING; PURIFICATION; RADIOIMMUNOASSAY; REACTIVITY; RECOMBINANT DNA; SENSITIVITY; STH
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BIOASSAY; DAYS LIVING RADIOISOTOPES; DNA; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PERFORMANCE TESTING; PITUITARY HORMONES; RADIOASSAY; RADIOISOTOPES; SYNTHESIS; TESTING; TRACER TECHNIQUES
Optional Information
- Notes
- 6 refs.