Determination of urinary tetrahydroaldosterone glucosiduronic acid by radioimmunoassay

A procedure is described for determining tetrahydroaldosterone 3-glucosiduronic acid in unprocessed human urine by radioimmunoassay using tritium. The procedure is simple, accurate and requires relatively little time. Data on cross reactivity, on within-assay and between-assay variability, and on reliability are given. Values for urinary excretion of tetrahydroaldosterone, aldosterone and sodium are given for a group of 72 normal individuals (35 M; 37 F) who were on ad libitum salt intake. The average excretion of tetrahydroaldosterone is 5.3 times as great as that of aldosterone and the range of deviation (in percent) from the mean value is the same for tetrahydroaldosterone as for aldosterone. (author)