Comparison of the methane-oxidizing capacity of landfill cover soil amended with biochar produced using different pyrolysis temperatures

Highlights: • Enriched MOB was inoculated to biochar-amended landfill cover soil (LCS). • In-situ CH₄ mitigation of biochar-amended LCS was increased up to 46%. • The cation exchange capacity of biochar peaks at a pyrolysis temperature of 400 °C. • Biochar produced at 400 °C promotes the growth of type II methanotroph most in LCS. -- Abstract: The in-situ mitigation of methane (CH₄) in landfill gas using landfill cover soil (LCS) is a cost-effective approach, but its efficiency needs to be enhanced. In this study, we incorporated an enriched methane-oxidizing bacteria (MOB) consortium into LCS and established four biochar-amended LCS groups with biochar produced at 300 °C (BC300), 400 °C (BC400), 500 °C (BC500), and 600 °C (BC600). The purpose was to evaluate the CH₄ oxidation capacity of biochar-amended LCS after inoculation with MOB and to investigate how the physicochemical properties of biochar that are influenced by the pyrolysis temperature affect the performance and microbial activity of biochar-amended LCS. It was found that a 15% volume ratio (representing a mass ratio of 2.49%–2.78%) for biochar amendment in LCS enhanced CH₄ removal efficiency, with the highest removal observed to be 46% for BC400-amended LCS compared to 30% for the original LCS. In addition, CH₄ adsorption by the biochar was not observed, and a 15% mass ratio for biochar in the LCS had no or a negative impact. Besides improving the water-holding capacity and gas permeability of LCS, other possible advantages of biochar amendment in terms of CH₄ oxidization include greater retention of nutrients, electron acceptors, and exchangeable cations, as well as introducing iron ions. It was also found that CH₄ oxidation capacity and the methanotroph activity of biochar-amended LCS did not continue to increase with higher pyrolysis temperatures, even though higher micropore volumes and surface areas were obtained at higher pyrolysis temperatures. From this study, BC400 was identified as the optimal choice for the best performance in terms of enhancing both the CH₄ oxidation capacity of the amended LCS and the growth of type II methanotroph Methylocystaceae, which can possibly be attributed to having the highest cation exchange capacity of the four biochars.