DNA damage of melanoma cells B16F0 treated with boron compounds after neutron irradiation

DNA damage can be assessed by the single cell gel electrophoresis(SCGE), alias, Comet assay; a fast, simple and sensitive technique. Within the last 20 years, the comet assay has been used to investigate primary DNA damage, such as double-strand breaks, single strand breaks, alkali-labile sites, incomplete repair sites and crosslingks. Therefore, comet assay has been applied in a great number of studies to investigate the early biological effects of DNA-damaging agents in environmental, occupational and pathological conditions or exposure to chemicals at the cellular level. From this reason, we investigated the DNA damage in melanoma cells B16F0 treated with boron compounds such as BPA and BSH after neutron irradiation in vitro