Molecular bases for radiation mutagenesis. Three year progress report, November 15, 1975--November 14, 1978

Studies on isolation and characterization of radiation products included: isolation and identification of cis- and trans-Thy glycols from the mixture of gamma irradiation products of Thy; identification of two isomers as radiation products from radiolysis of cytosine in aerated aqueous solutions and radiolysis of Thd in aerated aqueous solutions. Studies on synthesis of radiation products included: Stereospecific synthesis of cis- and trans-pyrimidine glycols; preparation of pyrimidine hydroperoxide; and synthesis of cis- and trans-1-carbamylimidazolidone-4, 5-diols. Studies on biological effects were conducted using human lymphocyte cultures, Chinese hamster cells, and root tip cells of Vicia faba. Effects of 6-TOOH on chromosomal aberrations, colony-forming ability, and unscheduled DNA synthesis were studied. Mutagenic effects of radiation products of Thy derivatives were investigated. Methods and procedures are described for UV-spectroscopic study, isolation of products, characterization of products, kinetic studies, mechanistic studies, and tracer studies