Published 1975
| Version v1
Report
DNA-specific labelling by deoxyribonucleoside 5'-monophosphates in Saccharomyces cerevisiae
Creators
- 1. Frankfurt Univ. (F.R. Germany). Arbeitsgruppe Mikrobengenetik
Description
Growth of 5'-dTMP low-requiring strains is inhibited by exogenous 5'-dGMP and 5'-GMP at concentrations higher than 5 x 10-4 M. Synthesis of nucleic acids ceases and cells remain fixed in their respective place in the cell cycle. At concentrations lower than 10-5 M deoxyribonucleoside 5'-monophosphates may be employed for radioactive labelling, the label being preferentially used for DNA synthesis. Affinity to DNA of the 5'-dNMPs is in the order of 5'-dAMPS > 5'-dGMP > 5'-dCMP > 5'-dUMP. DNA-specific label is achieved with 5'-dAMP when the medium is supplemented with adenine and deoxyadenosine. (orig.)
Abstract (German)
Das Wachstum von 5'-dTMP-Staemmen wird durch exogenes 5'-dGMP und 5'-GMP mit Konzentrationen ueber 5 x 10-4 M unterdrueckt. Die Nukleinsaeuresynthese wird unterbrochen, und die Zellen werden in ihrem jeweiligen Zustand im Zellzyklus fixiert. Bei Konzentrationen unter 10-5 M koennen Desoxyribonukleoxid-5'-Monophosphate zur radioaktiven Markierung verwendet werden; diese Markierung wird vorwiegend bei der DNS-Synthese verwendet. Die DNS-Affinitaet von 5'-dNMP's entspricht ungefaehr 5'-dAMP > 5'-dGMP > 5'-dCMP > 5'-dUMP. Eine DNS-spezifische Markierung kann mit 5'-dAMP erreicht werden, wenn das Medium mit Adenin und Desoxyadenosin versetzt wird. (orig./AK)Additional details
Publishing Information
- Imprint Pagination
- 7 p.
- Report number
- AED-Conf--75-549-002
Conference
- Title
- Conference on radiation and cellular control processes.
- Dates
- 6 Oct 1975.
- Place
- Giessen, F.R. Germany.
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 7244441
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Resource subtype / Literary indicator
- Conference, Non-conventional Literature
- Descriptors DEI
- ADENOSINE; AMP; AUTORADIOGRAPHY; BIOSYNTHESIS; CARBON 14 COMPOUNDS; CELL CYCLE; DNA; GUANOSINE; IN VIVO; SACCHAROMYCES CEREVISIAE; THYMIDINE; THYMINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS
- Descriptors DEC
- AZINES; CARBON COMPOUNDS; FUNGI; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; MICROORGANISMS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PLANTS; PURINES; PYRIMIDINES; RIBOSIDES; SACCHAROMYCES; SYNTHESIS; URACILS; YEASTS
Optional Information
- Notes
- 2 figs.; 4 tabs.; 11 refs. With abstract. Available from ZAED.