development of solid phase anti-T3 and anti-T4 coated beads for detection of T3 and T4 hormones

the thyroid secretes two significant hormones thyroxin and triiodothyronine, commonly called T4 and T3, respectively. both of these hormones have profound effect of increasing the metabolic rate of the body. in the present study a radioimmunoassay system was developed to detect T3 and T4 hormones. the solid phase anti-T3 and anti-T4 coated beads was prepared also the radiolabeled T3 and T4 was prepared using diiodothyronine (T2) and I125 with chloramine-T method. the system was developed and characterized according to the terms of accuracy, sensitivity, specificity, cross- reactivity and dilution effect. also the results of patient serum obtained by the developed system were compared with that of commercially used kit. from the obtained results it was found that the prepared system was closely similar to that of commercially used one