The effect of gamma irradiation on fish transaminase and rhodanese
Creators
- 1. National Atomic Energy Agency, Jakarta (Indonesia). Pasar Djumat Research Centre
- 2. Bandung Inst. of Tech. (Indonesia). Chemistry Dept.
Description
Investigations on glutamic oxaloacetic transaminase (GOT), glutamic pyruvate transaminase (GPT) and rhodanese of both unirradiated and irradiated chub mackerel (Rastrelliger neglectus) have been carried out. Fish were irradiated with doses varied from 0 to 5 kGy, at a dose rate of 2 kGy/hr. Fish extracts prepared in 0.01 M phosphate-buffer of pH 7 were used throughout the enzyme experiments. The conclusion drawn from five replicates showed a significant decrease (P<=0.01) in fish G0T, GPT and rhodanese specific activities after irradiation. It is proved that GOT and GPT were more susceptible towards irradiation as compared to rhodanese. An irradiation dose of 4 kGy was able to inactivate G0T, GPT and rhodanese for ca 50, 44 and 36% respectively. Evidently transaminase as well as rhodanese specific activities to spoiled fish were significantly lower (P<=0.01) than those of fresh fish. The residual GOT, GPT and rhodanese specific activities in spoiled fish was found to be about 35, 41 and 22% respectively. (author)
Additional details
Publishing Information
- Journal Title
- Majalah BATAN
- Journal Volume
- 16
- Journal Issue
- 1
- Series
- Majalah BATAN.
- Journal Page Range
- 75-82
- ISSN
- 0303-2876
INIS
- Country of Publication
- Indonesia
- Country of Input or Organization
- Indonesia
- INIS RN
- 15061302
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Resource subtype / Literary indicator
- Numerical Data
- Descriptors DEI
- AMINOTRANSFERASES; COBALT 60; DOSE RATES; EXPERIMENTAL DATA; FISHES; GAMMA RADIATION; GLUTAMIC ACID; INACTIVATION; OXALIC ACID; PYRUVIC ACID; RADIATION DOSES; THIOCYANATES
- Descriptors DEC
- AMINO ACIDS; ANIMALS; ANTITHYROID DRUGS; AQUATIC ORGANISMS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBONIC ACID DERIVATIVES; CARBOXYLIC ACIDS; COBALT ISOTOPES; DATA; DICARBOXYLIC ACIDS; DRUGS; ELECTROMAGNETIC RADIATION; ENZYMES; INFORMATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; KETO ACIDS; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUMERICAL DATA; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; RADIATIONS; RADIOISOTOPES; TRANSFERASES; VERTEBRATES; YEARS LIVING RADIOISOTOPES
Optional Information
- Notes
- 7 refs.