Published April 2019 | Version v1
Journal article Metadata-only

A rapid fluorometric method for determination of aflatoxin B1 in plant-derived food by using a thioflavin T-based aptasensor

Creators

  • 1. Tianjin University of Science and Technology, State Key Laboratory for Food Nutrition and Safety, College of Food Engineering and Biotechnology (China)
  • 2. The Biotechnology Research Institute of Chinese Academy of Agricultural Sciences (China)

Description

A fluorometric aptamer-based method is described for the determination of aflatoxin B1 (AFB1). The fluorescent dye thioflavin T (ThT) forms a complex with the aptamer against AFB1 (aptamer/ThT), and the fluorescence of the complex is strongly enhanced. On addition of AFB1, it will bind to the aptamer and release ThT. The fluorescence of free ThT is much weaker. The fluorescence of the system, best measured at excitation/emission wavelengths of 440/487 nm, drops gradually in the AFB1 concentration range from 0.2 to 200 ng·mL−1, exhibiting good linearity. The detection limits are 0.2 ng·mL−1 in buffer solution, and 1 ng·mL−1 when applied to plant-derived food. The recovery of AFB1 from spiked foodstuff ranges from 74.7% to 121%. The assay can be performed within 20 min.

.

Additional details

Identifiers

DOI
10.1007/s00604-019-3325-9;

Publishing Information

Journal Title
Mikrochimica Acta
Journal Volume
186
Journal Issue
4
Journal Page Range
p. 1-7
ISSN
0026-3672
CODEN
MIACAQ

INIS

Country of Publication
Austria
Country of Input or Organization
Austria
INIS RN
52000039
Subject category
S77: NANOSCIENCE AND NANOTECHNOLOGY; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Descriptors DEI
AFLATOXINS; AMINES; BENZOTHIAZOLES; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; FOOD; MAIZE; NANOCHEMISTRY; OLIGONUCLEOTIDES; PEANUTS; RICE
Descriptors DEC
ANTIGENS; AZOLES; CEREALS; CHEMISTRY; DNA; EMISSION; EMISSION SPECTROSCOPY; GRAMINEAE; HAZARDOUS MATERIALS; HETEROCYCLIC COMPOUNDS; LILIOPSIDA; LUMINESCENCE; MAGNOLIOPHYTA; MATERIALS; MYCOTOXINS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PHOTON EMISSION; PLANTS; SEEDS; SPECTROSCOPY; THIAZOLES; TOXIC MATERIALS; TOXINS

Optional Information

Copyright
Copyright (c) 2019 Springer-Verlag GmbH Austria, part of Springer Nature