Published 1982
| Version v1
Miscellaneous
Determination of the radiochemical purity of tritium-labelled nucleoside-5'-triphosphates using PEI-cellulose microcolumns
Description
The radiochemical purity of tritium-labelled nucleoside-5'-triphosphates was analysed by means of a highly sensitive continuous-operation detector of UV absorption and a PEI-cellulose microcolumn (3 mm in diameter, 130 mm long). Analysis was performed with 0.1-0.2 nucleotide optical density units (lambda = 254 nm). Elution was carried out by a NaCl concentration gradient. All nucleotides were separated under identical conditions. The duration of analysis did not exceed 1-2 hrs
Additional details
Additional titles
- Original title (Russian)
- Определение радиохимической чистоты меченных тритием нуклеозид-5′-трифосфатов с применением микроколонок с ПЭИ-целлюлозой
Publishing Information
- Imprint Title
- Radioisotope-labelled organic compounds. Part 2
- Journal Page Range
- p. 266-270.
- Report number
- INIS-mf--10548
Conference
- Title
- Radioisotope-labelled organic compounds.
- Dates
- 8-11 Dec 1981.
- Place
- Leningrad (USSR).
INIS
- Country of Publication
- International Centre for Scientific and Technical Information (ICSTI)
- Country of Input or Organization
- International Centre for Scientific and Technical Information (ICSTI)
- INIS RN
- 18021892
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- CELLULOSE; IMPURITIES; ION EXCHANGE CHROMATOGRAPHY; LEACHING; NUCLEOTIDES; OPACITY; ORGANIC PHOSPHORUS COMPOUNDS; RADIOACTIVITY; SENSITIVITY; TRITIUM COMPOUNDS
- Descriptors DEC
- CARBOHYDRATES; CHROMATOGRAPHY; DISSOLUTION; HYDROGEN COMPOUNDS; OPTICAL PROPERTIES; ORGANIC COMPOUNDS; PHYSICAL PROPERTIES; POLYSACCHARIDES; SACCHARIDES; SEPARATION PROCESSES
Optional Information
- Notes
- 3 figs.; 6 refs. Imprint:Organicheskie soedineniya, mechennye radioaktivnymi izotopami.;Materialy 2 Simpoziuma stran-chlenov SEhV, Leningrad, 8-11 dekabrya 1981 g. Chast' 2.