Comparative SPR study on the effect of nanomaterials on the biological activity of adsorbed proteins
- 1. Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai, 200444 (China)
- 2. Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029 (China)
- 3. Department of Nanomedical Engineering, Pusan National University, Miryang, 627-706 (Korea, Republic of)
Description
Bioactivity of proteins is evaluated to test the adverse effects of nanoparticles interjected into biological systems. Surface plasmon resonance (SPR) spectroscopy detects binding affinity that is normally related to biological activity. Utilizing SPR spectroscopy, a concise testing matrix is established by investigating the adsorption level of bovine serum albumin (BSA) and anti-BSA on the surface covered with 11-mercaptoundecanoic acid (MUA); magnetic nanoparticles (MNPs) and single-walled carbon nanotubes (SWCNTs), respectively. The immunoactivity of BSA on MNPs and SWCNT decreased by 18 % and 5 %, respectively, compared to that on the gold film modified with MUA. This indicates that MNPs cause a considerable loss of biological activity of adsorbed protein. This effect can be utilized for practical applications on detailed biophysical research and nanotoxicity studies. (author)
Additional details
Identifiers
Publishing Information
- Journal Title
- Microchimica Acta (Online)
- Journal Volume
- 178
- Journal Issue
- 3-4
- Journal Page Range
- p. 301-307
- ISSN
- 1436-5073
INIS
- Country of Publication
- Austria
- Country of Input or Organization
- Austria
- INIS RN
- 44011857
- Subject category
- S77: NANOSCIENCE AND NANOTECHNOLOGY; S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ADSORPTION; ALBUMINS; CARBON; CATTLE; GOLD; NANOTUBES; PARTICLES; SPECTROSCOPY; THIN FILMS
- Descriptors DEC
- ANIMALS; DOMESTIC ANIMALS; ELEMENTS; FILMS; MAMMALS; METALS; NANOSTRUCTURES; NONMETALS; ORGANIC COMPOUNDS; PROTEINS; RUMINANTS; SORPTION; TRANSITION ELEMENTS; VERTEBRATES