Structure of the hypothetical DUF1811-family protein GK0453 from Geobacillus kaustophilus HTA426
Creators
- 1. RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045 (Japan)
- 2. National Institute of Mental Health and Neuro Sciences (NIMHANS), Bangalore 560 029 (India)
- 3. University of Liverpool, Liverpool L69 7ZB (United Kingdom)
- 4. The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan)
- 5. Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan)
Description
The gene encoding the hypothetical DUF1811-family protein GK0453 from G. kaustophilus was cloned and expressed. The crystal structure of the protein was determined by the molecular-replacement method and was refined to 2.2 Å resolution. The crystal structure of a conserved hypothetical protein, GK0453, from Geobacillus kaustophilus has been determined to 2.2 Å resolution. The crystal belonged to space group P43212, with unit-cell parameters a = b = 75.69, c = 64.18 Å. The structure was determined by the molecular-replacement method and was refined to a final R factor of 22.6% (Rfree = 26.3%). Based on structural homology, the GK0453 protein possesses two independent binding sites and hence it may simultaneously interact with two proteins or with a protein and a nucleic acid
Availability note (English)
Available from http://dx.doi.org/10.1107/S1744309113003369; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614154Additional details
Identifiers
- URL
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614154;
- DOI
- 10.1107/S1744309113003369;
- PII
- S1744309113003369;
Publishing Information
- Journal Title
- Acta Crystallographica. Section F
- Journal Volume
- 69
- Journal Issue
- Pt 4
- Journal Page Range
- p. 342-345
- ISSN
- 1744-3091
- CODEN
- ACSFCL
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 46082004
- Subject category
- S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
- Descriptors DEI
- CRYSTAL STRUCTURE; CRYSTALS; PROTEINS; R FACTORS; RESOLUTION; SPACE GROUPS
- Descriptors DEC
- ORGANIC COMPOUNDS; SYMMETRY GROUPS
Optional Information
- Copyright
- Copyright (c) Padmanabhan et al. 2013
- Notes
- PMCID: PMC3614154; PMID: 23545635; PUBLISHER-ID: sw5059; OAI: oai:pubmedcentral.nih.gov:3614154; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.