Directed evolution as an approach to the design of efficient biocatalysts

Methodical approaches to the modification of the catalytic properties of enzymes by various means of directed evolution are summarised. A number of particular examples of the design of enzymes with new and enhanced properties, including higher thermal stability, solubility, substrate specificity, stereoselectivity, etc., are considered. The prospects for further development of the directed evolution strategy for enzymes and proteins with other functions are discussed.