Published February 1976 | Version v1
Journal article Metadata-only

The structure of teichoic acid from Bacillus subtilis var. niger WM as determined by 13C nuclear-magnetic-resonance spectroscopy

Creators

  • 1. Amsterdam Univ. (Netherlands). Lab. voor Microbiologie
  • 2. Amsterdam Univ. (Netherlands). Lab. voor Organische Scheikunde

Description

The walls of Bacillus subtilis var. niger WM, grown in a Mg2+-limited chemostat culture (carbon source glucose, dilution rate = 0.2 h-1, 370C, pH 7) contained 45% (w/w) teichoic acid, a polymer composed of glycerol, phosphate and glucose in the molar ratio 1.00 : 1.00 : 0.88. Alkaline hydrolysis of this teichoic acid yielded 1-O-β-glucosylglycerol phosphate (together with small amounts of glycerol phosphate), and 13C nuclear magnetic resonance spectra of this hydrolysis product, and its derivative after alkaline phosphatase treatment, confirmed that the monomeric unit was 1-O-β-glucosylglycerol-3-phosphate. Assignment of the resonances in the spectrum of undegraded teichoic acid revealed that the polymer was a poly[(2,3)glycerol phosphate], glucosidically substituted on C-1 of glycerol with β-glucose. (orig.)

Abstract (German)

Die Zellwaende von Bacillus subtilis var. niger WM aus einer Mg2+-limitierten Kultur (Kohlenstoffglukose, Verduennungsrate = 0,2 h-1, 370C, pH 7) enthielten 45% (w/w) Teichonsaeure, ein Polymer aus Glycerin, Phosphat und Glukose im molaren Verhaeltnis 1,00 : 1,00 : 0,88. Durch alkalische Hydrolyse dieser Teichonsaeure erhielt man 1-O-β-Glukosylglycerinphosphat (und kleine Mengen Glycerinphosphat); die 13C-NMR-Spektren dieses Hydrolyseprodukts und seines Derivats nach Behandlung mit alkalischer-phosphatase bestaetigten, dass es sich bei dem Monomer um 1-O-β-Glukosylglycerin-3-phosphat handelte. Die Resonanzzuordnung im Spektrum der nichtdegradierten Teichonsaeure zeigte, dass das Polymer ein Poly[(2,3)glycerin phosphat] mit glykosidischer Substitution am C-1 von Glycerin mit β-Glukose war. (orig.)

Additional details

Identifiers

DOI
10.1111/j.1432-1033.1976.tb10090.x;

Publishing Information

Journal Title
European Journal of Biochemistry
Journal Volume
62
Journal Issue
1
Series
Eur. J. Biochem.
Journal Page Range
1-6
ISSN
0014-2956

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
7270069
Subject category
S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Descriptors DEI
BACILLUS SUBTILIS; BIOCHEMISTRY; CARBON 13; CELL CULTURES; CELL WALL; HYDROLYSIS; METABOLISM; MOLECULAR BIOLOGY; NUCLEAR MAGNETIC RESONANCE; STRUCTURAL CHEMICAL ANALYSIS
Descriptors DEC
BACILLUS; BACTERIA; CARBON ISOTOPES; CELL CONSTITUENTS; CHEMICAL REACTIONS; CHEMISTRY; DECOMPOSITION; EVEN-ODD NUCLEI; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MICROORGANISMS; NUCLEI; RESONANCE; SOLVOLYSIS; STABLE ISOTOPES

Optional Information

Notes
3 figs.; 3 tabs.; 17 refs.; Updated automatically by Metadata and Full-Text Enrichment Agent