Changes in cytogenetic indicators during the application of compounds of the copper-organic complexes

One of the priorities of the contemporary radiobiology is the search for new efficient radioprotective compounds. In this field metal-organic complexes of high antioxidant activity are of special interest. Such complexes have low toxicity and obvious radioprotective properties. With a view to relieve possible radiation protective actions, we studied compounds of copper-organic complexes: [Cu (PTA)₄] BF₄ and[Cu(PCN)(HBP₂₃)]BF₄, in which legands are: PTA-1,3,5-triaza-7-phosphaadamantane and PCN-tris(cyanoethyl)phosphine HBP_z3-trispyrazolilborato (hereinafter referred to as ''PTA" and ''PCN'') which were synthesized in Camerino University (Italy) under the supervision of Professor Carlo Santini. The experiments were carried out on reproductive, white, outbred rats with middle-weight of 180-200gr. An hour before general, single-shot radiography of the animals with dose 5,3Gy and 7,0Gy, by PYM17 equipment, PTA and PNC Cu-organic complexes were injected abdominally with dose of 20mg/kg in the form of an aqueous suspension. Since ionizing radiation is essential disturbing factor of cytogenetic status of the organism, we analyzed radiation-induced clastogenesis of the bone marrow cells (BMC) as one of the most informative indicators. Generally accepted method of cytogenetic analysis of white rats' BMC indices was used. Proliferative activity (mitotic index-MI), chromosome aberration (CA), cell ploidy (CP) was taken into consideration. We also performed hematological analysis of peripheral blood: red blood cell count, leucocyte count, level of hemoglobin. Basing on the survival results, cytogenetic and hematological indicators, it can be concluded that study complexes demonstrate noticeable radioprotective properties. Both complexes showed identical effects on experimental animals according to all observed criteria. In eruptive phase, on early dates of analysis (3^rd and 7^th days) both compounds soften the damaging effects of radiation with both doses which is especially obvious in significant shifts of mitotic index and CA in comparison with control data. On the last dates of observation (14^th and 30^th days) many data of analysis (MI, red blood readings) approached the standard data (norm), CA and number of leucocytes in comparison with the control data, demonstrate statistically significant difference. Based on the results obtained, it can be assumed that the studied Cu-ligands effectively promote reparative processes in bone marrow cells and have the properties of pronounced radiomodifiers.