Ku gene mutation of radiosensitive mouse mammary carcinoma-derived cell line SX-9
- 1. Institute of Radiation Medicine Beijing (China)
Description
Objective: To investigate the ku gene status of radiosensitive cell line SX-9 and the effects of ku80 gene mutation on radiosensitivity of SX-9. Methods: After comparing the ku70/80 gene status of SX-9 to SR-1 with molecular cloning, the survival fraction of SX-9 transfected with wild-type human ku80, SX-9 and SR-1 was investigated by clonogenic assay. Results: Radiosensitive mouse mammary carcinoma-derived cell line SX-9 bore mutation in ku80 gene, and its survival fraction after γ-ray irradiation could be restored by transfection of wild-type human ku80 gene. Conclusions: There are two mutation sites in ku80 gene in radiosensitive mouse mammary carcinoma-derived cell line SX-9, which could be the cause of its deficiency in repair of DNA double strand breaks
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 20
- Journal Issue
- 4
- Journal Page Range
- p. 242-244
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 32003428
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BIOLOGICAL REPAIR; CARCINOMAS; DNA; GAMMA RADIATION; GENE MUTATIONS; MAMMARY GLANDS; MICE; RADIATION INJURIES; RADIOSENSITIVITY; SURVIVAL CURVES; TUMOR CELLS
- Descriptors DEC
- ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BODY; DISEASES; ELECTROMAGNETIC RADIATION; GLANDS; INJURIES; IONIZING RADIATIONS; MAMMALS; MUTATIONS; NEOPLASMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; RADIATION EFFECTS; RADIATIONS; REPAIR; RODENTS; VERTEBRATES