Published 1976 | Version v1
Book Metadata-only

Parathyroid hormone (PTH), ch. 19

Creators

  • 1. Mount Sinai School of Medicine, New York (USA)
  • 2. Veterans Administration Hospital, Bronx, N.Y. (USA)

Description

The radioimmunoassay of PTH and the complications arising from both the relatively low sensitivity of most of the antisera being used and the hormonal heterogeneity are discussed. After labelling of PTH with 125I by the chloramine-T method, the labelled hormone is purified by QUSO granules. The requirements for the assay including a sample protocol for the incubation mixtures are described. The bound and free hormones are separated by adsorption, using talc, charcoal or QUSO pellets

Additional details

Publishing Information

Publisher
North-Holland.
Imprint Place
Amsterdam, Netherlands
ISBN
0 7204 0630 7
Imprint Title
Methods in radioimmunoassay of peptide hormones
Imprint Pagination
p. 181-186.

INIS

Country of Publication
Netherlands
Country of Input or Organization
Netherlands
INIS RN
8301325
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ADSORPTION; AFFINITY; GLASS; PARATHORMONE; RADIOIMMUNOASSAY
Descriptors DEC
HORMONES; ISOTOPE APPLICATIONS; PEPTIDE HORMONES; TRACER TECHNIQUES

Optional Information

Notes
Imprint:Reprinted from Methods in Investigative and Diagnostic Endocrinology, vols. 2A and 2B, 1973, North-Holland.