Published August 2004 | Version v1
Journal article Metadata-only

Effects of brachytherapy on gene expressions of elastin and elastase

Creators

  • 1. Three Gorges Univ., Yichang (China). Renhe Hospital, Dept. of Cardiology

Description

Objective: To study the effects of brachytherapy on the gene expressions of elastin and elastase in cultured rat vascular smooth muscle cells (VSMCs). Methods: Rat VSMCs cultured in DMEM containing 10% FBS were irradiated by 60Co γ-rays at 0, 7, 14, 28 Gy respectively. Then mRNA levels of elastin and elastase were determined by reverse transcription competitive PCR(RT-PCR). Results: Brachytherapy inhibited the expressions of elastase. Elastase mRNA decreased 25.3% and 50.1% in VSMC irradiated with 14, 28 Gy, respectively (P<0.05). The elastin mRNA level increased 80.7% and 102.3% in VSMC irradiated with 14, 25 Gy, respectively (P<0.05). Conclusion: Brachytherapy inhabits the expressions of elastase and increased elastin in VSMC cells

Additional details

Publishing Information

Journal Title
Chinese Journal of Radiological Medicine and Protection
Journal Volume
24
Journal Issue
4
Journal Page Range
p. 342-344
ISSN
0254-5098

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
36053122
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
BIOLOGICAL RADIATION EFFECTS; BIOSYNTHESIS; BLOOD VESSELS; BRACHYTHERAPY; ENZYMES; INHIBITION; LOW DOSE IRRADIATION; MESSENGER-RNA; PROTEINS; RADIATION DOSES
Descriptors DEC
BIOLOGICAL EFFECTS; BODY; CARDIOVASCULAR SYSTEM; DOSES; IRRADIATION; MEDICINE; NUCLEAR MEDICINE; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIATION EFFECTS; RADIOLOGY; RADIOTHERAPY; RNA; SYNTHESIS; THERAPY