Effects of chilling on protein synthesis in tomato suspension cultures
Description
The effect of chilling on cell growth, cell viability, protein content and protein composition in suspension cultures of L. esculentum and L. hirsutum was investigated. Cell growth for both species was arrested at 2 degrees C but when cultures were transferred to 25 degree C cell growth resumed. There was no difference in viability between control and chilled cultures of L. esculentum, however, L. hirsutum control cultures exhibited larger amounts of Fluorescein Diacetate induced fluorescence than chilled cultures. 35S-methionine incorporation into proteins was 2.5-2 times higher in L. hirsutum than in L. esculentum. Quantitative and qualitative differences, in 35S-methionine labelled proteins, between chilled and control cultures were observed by SDS-PAGE and fluorography. Protein content in chilled cultures decreased over time but then increased when cultures were transferred to 25 degrees C
Additional details
Publishing Information
- Journal Title
- Plant Physiology, Supplement
- Journal Volume
- 89
- Journal Issue
- 4
- Series
- Plant Physiol., Suppl.
- Journal Page Range
- 31
- ISSN
- 0079-2241
- CODEN
- PPYSA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 21090107
- Subject category
- S61: RADIATION PROTECTION AND DOSIMETRY; S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BIOSYNTHESIS; CELL CULTURES; ELECTROPHORESIS; GENETIC VARIABILITY; HERBS; METHIONINE; PLANT GROWTH; PROTEINS; SULFUR 35; TEMPERATURE DEPENDENCE; TOMATOES; TRACER TECHNIQUES
- Descriptors DEC
- AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL VARIABILITY; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DRUGS; EVEN-ODD NUCLEI; FOOD; GROWTH; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LIPOTROPIC FACTORS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PLANTS; RADIOISOTOPES; SULFUR ISOTOPES; SYNTHESIS; VEGETABLES